Diagnosing Male Genitourinary Conditions

The P.E.U.S. – Post Ejaculate Urine Sample – Protocol

The Post Ejaculate Urine Sample (PEUS) protocol represents an advanced yet experimental approach to diagnosing male genitourinary conditions. Utilizing cutting-edge next-generation sequencing (NGS) technology, this protocol aims to identify a comprehensive range of pathogens, including bacteria, viruses, fungi, and parasites, that traditional diagnostic methods might miss. However, due to its experimental nature, the PEUS protocol may not be suitable for all situations. Patients are strongly advised to follow the guidance of their physicians and laboratories when considering this method. The PEUS protocol is particularly recommended for use when standard tests fail to detect the causative agents of persistent or complex genitourinary symptoms.

With the advancement of technology, diagnosing male genitourinary conditions has become more precise and tailored to individual patient needs. One such advancement is the availability of specialized tests designed to identify a wide range of pathogens, offering a comprehensive approach to diagnosis and treatment.


Several laboratories now offer DNA tests specifically tailored to identify pathogens associated with male genitourinary conditions. These tests utilize next-generation sequencing (NGS) technology and advanced analytic algorithms to detect bacteria, viruses, fungi, and parasites with high accuracy. Keywords such as “Infectious Disease Genetic Testing” and “NGS” can help you and your physician identify which labs might work best for you.

Example Laboratories:

What follows is the step-by-step guide to acquiring the test and the actual protocol necessary to complete the test. Please note that the test is designed specifically for men with genitourinary symptoms.

Order the Kit

In most cases, patients or physicians can now easily order these specialized tests directly from the laboratory’s website. The ordering process typically involves selecting a kit that offers an NGS of urine samples. In some cases, a doctor’s approval may be required, especially for patients residing in the United States.

Tips For a Great Result

At Least Three Days Before the Test

Cease consumption of any antiparasitics, antibiotics, antifungals, antivirals, or anything that would otherwise damage pathogens. This includes any and all drugs and/or supplements. However, it can be speculated that you can increase your odds of receiving viable test results by discontinuing such substances earlier.

The Day Before

While not entirely necessary, it may be wise to massage the impacted testicle, prostate, and/or tissues several times throughout the day. It is theorized that this will help with the release and motility of some organisms. 

Sample Collection

Ideally, you want to be as clean as possible. Cross-contamination hinders discovery.  

  1. Wash and sanitize your hands.
  2. Put on nitrile gloves if they are available or provided.
  3. Wash and sanitize your penis using the towelette that is provided.
  4. Without lube, provide pleasurable friction on the head/glans of your penis, which leads to ejaculation.
  5. Cap the collection cup tightly.

NOTE: Allow the body to expel the semen with minimal manual assistance, and ensure that you do not scrape the penis against the cup. You will want the semen to go straight from the urethra to the cup without any other contact or interference.

NOTE: You can massage the testicles or prostate area during this process. Doing so will force a little more semen to be expelled.

Your kit will likely include a small piece of parafilm. It might look like trash, so be careful not to discard it. If you have one, you will separate the parafilm from the backing and stretch and wrap the parafilm horizontally around the cap of the cup. This will help to prevent sample loss when shipped. 


  1. Follow the instructions regarding the sample and the shipping container.
  2. Follow the shipping instructions.

Advanced Measures (For Medical Staff)

Healthcare professionals analyzing samples may choose to include artifacts identified under the microscope in the test. This can be done by transferring artifacts to the urine sample using a micropipette.

Medical professionals are invited to explore the rationale behind the PEUS Protocol by CLICKING HERE.

Interpreting Test Results

Upon completion of the test, the infectious disease report will be provided to the healthcare provider. Patients living outside the United States may receive their own report. While laboratories may offer consultations to explain test results, they cannot provide treatment guidance or prescribe medications.

Yes… it works!

Here are just a few of the reported potentially pathogenic organisms discovered using this method:

  • Bacteria
    • Corynebacterium pseudogenitaliumCurrently the Most Dominate Species Discovered
    • Gardnerella vaginalis – Second Most Dominant Species Discovered
    • Cutibacterium acnes
    • Corynebacterium jeikeium
    • Corynebacterium minutissimum
    • Porphyromonas bennonis
    • Corynebacterium singulare
    • Streptococcus agalactiae
    • Dermabacter vaginalis
    • Finegoldia magna
    • Brevibacterium paucivorans
    • Tessaracoccus timonensis
    • Corynebacterium aurimucosum
    • Corynebacterium kefirresidentii
    • Corynebacterium appendicis
    • Peptoniphilus harei
    • Streptococcus mitis
    • Corynebacterium tuberculostearicum
    • Pseudoclavibacter alba
    • Anaerococcus provencensis
    • Brevibacterium mcbrellneri
    • Dermabacter hominis
    • Peptoniphilus lacrimalis
  • Fungi
    • Malassezia restricta
  • Viruses
    • Propionibacterium virus MrAK

If you would like to add your findings upon completion, please use the contact form. Your name will not be included. We are only interested in the organism(s) found. Thank you.

Conflict of Interest Statement: Dr. David Robertson has NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, or affiliations) in the lab, or materials discussed in this protocol.


The scientific validity of this is still under investigation. Know the risks and potential benefits of experimental protocols and talk to your healthcare provider before participating if you are unsure. Choosing to participate in and or otherwise use this information is an important personal decision. Before you participate in and or use this information, discuss all options with your healthcare provider and other trusted advisors.

Dr. Robertson is a health researcher and educator, not a physician. The information provided here is not medical advice, a professional diagnosis, opinion, treatment, or service to you or any other individual. The information provided is for educational and anecdotal purposes only and is not a substitute for medical or professional care. You should not use the information in place of a visit, call consultation, or the advice of your physician or other healthcare providers. Dr. Robertson is not liable or responsible for any advice, course of treatment, diagnosis, or additional information, services, or product you obtain or utilize. IF YOU BELIEVE YOU HAVE A MEDICAL EMERGENCY, YOU SHOULD IMMEDIATELY CALL 911 OR YOUR PHYSICIAN.